响应面法优化碱性蛋白酶提取榛子油工艺.pdf

CHINA OUS AND FATS
201lvol.36Na.12
新油源
响应面法优化碱性蛋白酶提取榛子油工艺
王腔男,江连洲,李杨2,李丹丹',刘珙,王梅
(1.东北农业大学食品学院,哈尔滨15002.国家大豆エ程技术研究中心,哈尔滨150030)
摘要:采用 Alcalase藏性蛋白酶水酶法提取榛子油,以提油率为指标,对影响提油率的各个因素进
行了研究,并用响应面法倪化了提油エ艺。F检验可以得到因素影响大小顺序为:酶解时间>加酶
量>料液比>酶解温度>酶解pH。得到优化酶解条件为:加酶量1.6%,酶解温度51℃,酶解时间
1.9h,料液比1:56,酶解pH10。在优化酶解条件下,榛子提油率可达92.92%。同时测得榛子油
的棕榈酸含璗4.33%,油酸67.10%,亚油酸0.09%,亚麻酸0.09%,硬脂酸1.165%。
关键词:水酶法; Alcalase碱性蛋白酶;榛子油;响应面法;脂肪酸
中图分类号:TS225;TQ644文献标志码:A文章编号:1003-7969(20112-006-04
Optimization on Alcalase extraction conditions of hazelnut oil
by response surface methodology
WANG Shengnan, JIANG Lianzhou*, LI Yang
LI Dandan, LIU Qi, WANG Mei
( 1. College of Food Science, Northeast Agricultural University, Harbin 150030, China
2. The National Research Center of Soybean Engineering and Technology, Harbin 150030, China
Abstract: Hazelnut oil was extracted from hazelnut kemel by aqueous enzymatic mcthod. The effects of dif-
ferent factors on the total extraction rate of hazelnut oil were studied. Then, response surface methodology
was applied lo optimize the extraction conditions. The effeets of five factors on oil extraction rate in descend-
ing order were hydrolysis time enzyme(Alcalase )amount> ratio of solid to liquid> hydrolysis tempera
ture PH The optimum conditions were: enzyme amount 1.6%, hyrolysis temperature 51 C, time 1.9h
ratio of solid to liquid 1: 5.6, PH 10. Under the optimum condition, the total extraction rate of hazelnut
oil eace 292. The content of fatty acid in hazelnut oil were as follows palmitic acid 4. 33%, oleic
acid 67. 10%, linoleic acid 0.09 linolenic acid 0.09% stearic acid 1 165
Key words: aqueous enzymatic extraction; Alcalase; az nut oil; esponse surface methodology; fatty acid
榛子( Corylus heteroph ylla Fisch. exbess),又名棰发生反应,最大优点就是能同时利用油料的油脂与蛋
子、平榛、山反粟等,属桦木科植物的种仁,为野生,榛白,是当前国内外食品工业研究的热点?。
子果仁味美可口,营养丰富。榛子油为干性油,色
本实验利用 Alcalase碱性蛋白酶水解榛子仁提
清味香,其中油酸含量可达82.1%,为优质食用取榛子油,采用单因素实验和响应面法对水解工.艺
油2]。水酶法作用条件温和(常温、无有机溶剂、无剧进行了优化,并对提取的榛子油脂肪酸组成及含量
烈化学反应),体系中的降解产物一般不会与提取物进行了分析和测定,为水酶法提取榛子油提供了
定的理论依据。
收稿日期:2011-05-13
材料与方法
基金项目;黑龙江省攻关项日(GAO9B401-6);农业部现代农
1.1原料、试剂
业产业技术体系建设项目( nyet-004
作者简介:王胜男(1988),女,在读硕土,研究方向为粮食、
榛子仁,长白山野生平榛; Alcalase碱性蛋白酶
油脂及植物蛋白工.程(E~mail)ying198854@163.com
活力1.2x10°U/mL),丹麦Novo公司;乙醚、盐
通信作者:江连洲,教授博上生导师(E-mi) illam@13.om。酸、NaOH等均为分析纯
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